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| Menezes,Kelly Alexsandra Souza; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Martins,Lindete Míria Vieira; Fernandes Júnior,Paulo Ivan. |
| ABSTRACT Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from “mulungu” (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological nitrogen fixation; “mulungu”; Tree legumes; “Caatinga”; Rhizobia. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100404 |
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| Rodrigues,Dalila Ribeiro; Silva,Aleksandro Ferreira da; Cavalcanti,Maria Idaline Pessoa; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Ribeiro,Paula Rose de Almeida; Ferreira Neto,Reginaldo Alves; Freitas,Ana Dolores Santiago de; Fernandes-Júnior,Paulo Ivan. |
| Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological nitrogen fixation; "Mulungu"; Diversity; Inoculant; 16S rRNA. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300503 |
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